ISO100 Protein Review: 6 Hard Facts About Dymatize's Hydrolyzed Whey Isolate
ISO100 PROTEIN REVIEW: 6 HARD FACTS ABOUT DYMATIZE'S HYDROLYZED WHEY ISOLATE
ISO100 protein from Dymatize is a hydrolyzed whey isolate. One 32 g scoop gives 25 g of protein, 2.6 g of leucine and 120 calories. Sugar and fat are 1 g each. There is no whey concentrate in the formula.
This Dymatize ISO 100 review reads the current label line by line, then checks the claims against research. Label figures come from the Dymatize product page; study figures come from PubMed abstracts, all listed at the end. For more products, see evidence based supplements.
ISO100 protein in three numbers
Three label numbers sum up ISO100 protein: how much protein a scoop gives, how many calories it costs, and how much leucine it carries.
Per 32 g scoop of Gourmet Chocolate
That is 78 percent protein by weight, our own sum from the label: 25 g in a 32 g scoop (Dymatize label).
Per scoop, with 2 g carbs and 1 g fat
Sugars are 1 g with 0 g added sugars (Dymatize label).
The amino acid that switches on muscle building
The position stand on protein suggests 700 to 3,000 mg of leucine per serving (PMID 28642676).
Label figures come from the Dymatize product page for Gourmet Chocolate, checked on 27 October 2026; other flavors differ slightly. Study figures come from PubMed abstracts. We do not sell this product and take no payment for reviews.
What this ISO100 protein review covers
The full ISO100 label: macros, amino acids, every ingredient and the allergen line.
The hydrolyzed claim: what research says about speed and muscle building.
Testing and value: what the banned substance claim means, and who should buy it.
Not covered
Prices. They change weekly; we show how to compare cost per gram instead.
Taste rankings. Those are personal. This is a label and evidence review.
The ISO100 protein label, line by line: macros per scoop
Here is the ISO100 protein label. These are the Supplement Facts for Gourmet Chocolate, one scoop of 32 g, as listed by Dymatize (Dymatize label).
| Nutrient | Per scoop | Read as |
|---|---|---|
| Calories | 120 | About 100 of them from protein (our math: 25 g x 4 kcal) |
| Protein | 25 g | 78 percent of the scoop by weight |
| Carbohydrate | 2 g | Under 1 g fiber, 1 g sugars, 0 g added |
| Fat | 1 g | 0 g saturated, 0 g trans |
| Cholesterol | 10 mg | Normal for a milk protein |
| Sodium | 180 mg | From salt in the formula |
| Potassium | 230 mg | Includes added potassium chloride |
| Leucine | 2.6 g | The amino acid table on the same page lists 2.698 g |
| BCAAs | 5.5 g | Leucine, isoleucine and valine combined |
One honest note: the Dymatize page gives 2.6 g leucine in its summary and 2.698 g in its amino acid table. Both round to the same practical answer.
ISO100 ingredients: what each one does
The ISO100 ingredients for Gourmet Chocolate, in label order (Dymatize label):
| Ingredient | What it does |
|---|---|
| Hydrolyzed whey protein isolate | Main protein; listed first, so it is the largest part |
| Whey protein isolate | Second protein source, not hydrolyzed |
| Cocoa powder, processed with alkali | Chocolate flavor |
| Salt (less than 2 percent) | Flavor; part of the 180 mg sodium |
| Soy lecithin (less than 2 percent) | Helps the powder mix; the reason for the soy allergen line |
| Natural and artificial flavor | Taste |
| Potassium chloride | Mineral and flavor |
| Sucralose, steviol glycosides | Zero calorie sweeteners |
The allergen statement reads: contains milk and soy (Dymatize label). There is no added creatine, glycine or taurine on the label, so the 25 g of protein comes from the two whey sources.
The ISO100 protein label does not give the split between hydrolyzed and regular isolate, or the degree of hydrolysis. That is normal for the category, but it means hydrolyzed is a processing claim you cannot measure from the tub.
Hydrolyzed whey: does it build more muscle?
Hydrolysis is the headline ISO100 protein claim. It cuts protein into shorter pieces before you drink it. Dymatize markets this as fast absorption (Dymatize label). The research supports faster, with a catch.
In 10 older men, a casein hydrolysate was absorbed 27 percent faster than the same intact casein. It raised blood amino acids 25 to 50 percent more (PMID 19474134).
Muscle building in that study was only a trend, not a clear win: 0.068 versus 0.054 percent per hour, P = 0.10 (PMID 19474134).
Whey itself matters more than hydrolysis. In 48 older men, 20 g of whey beat both casein and casein hydrolysate for muscle building (PMID 21367943).
In young men after training, whey hydrolysate raised muscle building about 122 percent more than casein and 31 percent more than soy (PMID 19589961).
No study we found tested ISO100 protein itself as a product, or hydrolyzed whey isolate against regular whey isolate in trained men.
Is one scoop of ISO100 protein enough?
After training, muscle building in young men peaked at 20 g of protein; 40 g added no more (PMID 19056590). One 25 g scoop of ISO100 protein clears that.
The protein position stand suggests 20 to 40 g per serving, with 700 to 3,000 mg of leucine (PMID 28642676). A scoop gives 2.6 g (Dymatize label).
| Target | Research value | One scoop |
|---|---|---|
| Protein per serving | 20 to 40 g (PMID 28642676) | 25 g |
| Leucine per serving | 700 to 3,000 mg (PMID 28642676) | 2.6 g |
| Daily protein, training | 1.4 to 2.0 g/kg (PMID 28642676) | Part of the day |
| Where gains stop rising | About 1.6 g/kg a day (PMID 28698222) | Part of the day |
Daily total matters more than timing. A meta-analysis found total protein intake was the strongest predictor of muscle growth, not when it was eaten (PMID 24299050).
ISO100 testing: what banned substance tested means
Testing is a big part of the ISO100 protein pitch. The Dymatize product page says Banned Substance tested, without naming the program (Dymatize label).
ISO100 Hydrolyzed Protein Powder (USA) appears on the Informed Choice certified products search (Informed Choice listing). Informed Choice is a third party program that tests supplements for substances banned in sport.
Certification lowers the risk of contamination for tested athletes. It does not prove the protein builds more muscle, and it does not cover every batch of every flavor in every country.
Is ISO100 protein worth it?
Is ISO100 protein worth the money? Prices change too often to print. Use this formula instead: price of the tub divided by servings, divided by 25 g. That is your cost per gram of protein.
Rule of thumb: compare that number with any other whey isolate. At matched protein and leucine, the muscle building effect is set mostly by the whey, not the brand (PMID 21367943).
| Fits you if | Look elsewhere if |
|---|---|
| You want a lean shake: 25 g protein for 120 calories | Cost per gram is your only metric |
| Whey concentrate upsets your stomach (isolate only) | You avoid sucralose or soy lecithin |
| You compete in tested sport and want a certified product | You need calories, not just protein |
| You want a fast protein after training | You want a slow protein before bed |
Before bed, the position stand names 30 to 40 g of casein for overnight muscle building (PMID 28642676). For a bottled shake, see the MRE protein shake review; for a bar, see the David protein bar review.
6 hard facts about ISO100 protein
1. One 32 g scoop gives 25 g protein and 120 calories. With 2 g carbs and 1 g fat (Dymatize label).
2. It is isolate only. Hydrolyzed whey isolate and whey isolate, no concentrate (Dymatize label).
3. Hydrolysis speeds absorption. A hydrolysate was absorbed 27 percent faster than intact protein, but muscle gains were only a trend (PMID 19474134).
4. One scoop clears the 20 g dose that maxed out muscle building after training (PMID 19056590).
5. It contains milk and soy, plus sucralose. (Dymatize label)
6. It is listed by Informed Choice. The tub itself only says banned substance tested (Informed Choice listing).
ISO100 protein: frequently asked questions
Is ISO 100 protein good?
Yes for a lean, fast protein: 25 g protein, 120 calories, 2 g carbs and 1 g fat per scoop, isolate only (Dymatize label). It is not cheaper per gram than a regular whey isolate.
What are the ISO100 ingredients?
Hydrolyzed whey protein isolate, whey protein isolate, cocoa, salt, soy lecithin, natural and artificial flavor, potassium chloride, sucralose and stevia in Gourmet Chocolate. It contains milk and soy (Dymatize label).
How much leucine is in ISO100?
2.6 g per scoop in the Dymatize summary, with 5.5 g BCAAs (Dymatize label). The protein position stand suggests 700 to 3,000 mg per serving (PMID 28642676).
Does hydrolyzed whey build more muscle?
It is absorbed faster. A hydrolysate was absorbed 27 percent faster than intact protein, but muscle building was only a trend (PMID 19474134).
Is ISO100 third party tested?
Dymatize says banned substance tested (Dymatize label). ISO100 Hydrolyzed Protein Powder (USA) appears on the Informed Choice certified products search (Informed Choice listing).
Is ISO100 good for lactose intolerance?
It is an isolate filtered to remove excess lactose, according to Dymatize (Dymatize label). No lactose figure is printed, so test your own tolerance.
How much ISO100 should I take a day?
Enough to reach your daily total. Gains stopped rising around 1.6 g of protein per kg a day (PMID 28698222). One scoop after training clears the 20 g dose (PMID 19056590).
When should I drink ISO100 protein?
Total daily protein predicted growth better than timing (PMID 24299050). After training is a sensible default.
Sources and further reading
Every figure on this page comes from one of the 9 sources below. That is the Dymatize product page, the Informed Choice listing, and 7 papers checked against PubMed on 27 October 2026. Percent by weight and calories from protein are our own arithmetic from the label. Reference links are dofollow to support open science.
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Final Educational Note
This review is educational and is not medical or nutrition advice. Label figures come from the Dymatize product page on the date shown and may change; always read the tub you buy. People with milk or soy allergy should not use this product.
MuscleScience does not sell supplements and is not paid for reviews. More guides sit on the supplements hub. Read more on the about page and in the full disclaimer.
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